Progesterone and Androstenedione Are Important Follicular Fluid Factors Regulating Porcine Oocyte Maturation Quality

Oocytes matured in vitro are useful for assisted human and farm animal reproduction. However, the quality of oocytes is usually lower than that vivo oocytes, possibly due to absence some important signal regulators vitro. In this study, untargeted metabolomics was used detect changes metabolites follicular fluid (FF) during pig oocyte maturation culture medium maturation. Our results showed total metabolite changing profile FF different from medium, but levels 23 differentially expressed (DEMs) changed by following same trend both These may be porcine We found progesterone androstenedione, two factors ovarian steroidogenesis pathway enriched DEMs, were upregulated The these 31 20 fold, respectively, they higher at mature stage. supplementation androstenedione significantly improved rate subsequent embryo developmental competence. finding suggests a metabolic abnormality affects oocytes. This study identified regulate their potential, which will helpful improve